With your raw or processed sperm sample, take your averaged reading with the iSperm.
Write down your total concentration for the sample (in Million/mL)
Multiply the percentage of progressively motile by the total concentration
Then, multiply that resulting number by the percentage morphologically normal
Divide 200 by that number
The number after line 5 is the minimum mL of that sample you are recommended to inseminate with, pending the sperm passes the checks listed above for progressive motility, concentration, and morphology.
For example, let's say we have a concentration of 450 M/mL (million per milliliter).
The results show progressive motility of 78%. So we multiply 450M/mL by 78% (or 0.78). It is 351.
Next, we have identified that most if not all appear totally normal in shape and size (morphology). Let's give it a 95%. 351 x 0.95 = 333 (Million/mL healthy sperm)
200/333 =0.60 So we have a minimum sample volume of 0.6mL.
If we deposit 10mL AI, we have 333 Million/mL x 10mL = 3,330 million total healthy sperms in that 10mL sample, well over the recommended minimum 200 million total..
However, remember if you are using an extender, or freezing/thawing the sperm for later use, that motility will generally decrease, and the extender will DILUTE that value. So your 333 million per milliliter, mixed with two parts extender, is now 111 million per milliliter.
Recommended Sperm result values
For best results, your sperm sample is recommended to have the below-listed parameters or better
1.70% or greater forward progressive motility
2.90% or greater normal morphology
3.200 million total motile, healthy sperm per insemination
4.300 million to 2 billion total sperm count per ejaculation
Since the sperm may encounter too many collisions or be too optically condensed above 75 M/mL, the velocity parameters, tracking function, and progressive motility will be activated only when the concentration is below 75 Million/ml (you may refer to the "Specifications" part of the iSperm manual or the following graph)The range that enables reporting of Progressive Motility will be 10-75 M/mL, optimized at 30-60M/mL. Please reference the following chart when planning the dilution necessary to bring the sample into this range.